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Many of these alleles were also present in RL The F1 was used to make a population of doubled haploid DH lines. A selection of lines from the DH population was used to create a provisional genetic map of the A. Lr32 conferred resistance to different isolates of Puccinia triticina obtained from wheat fields in Canada between and Singh 3 reported no case of virulence on Lr32 among isolates collected in Mexico between and and no virulence has Countersink Drill Bit Sizes 31 been noted in Australia 4.
Pretorius and Bender 5 demonstrated virulence on Lr32 in Japanese Wood Carving Tools Set Size a rare virulence phenotype isolated from triticale in the western Cape South Africa.
Effect of Lr32 on Agronomics and Quality. Both Lr32 -carrying lines, BW and RL, were tested on the field for effects of the leaf rust resistance gene on a series of agronomic and quality related traits. The introduction of Lr32 in Katepwa had no significant effect on the traits assessed, except for small reduction in plant height and test weight and an increase in kernel size.
The effect of Lr32 in Thatcher was more marked, there were increases in yield, straw strength, test weight, kernel size and kernel hardness. Some of these changes could be pleiotropic effects caused by the later maturity of RL compared to Thatcher. Thomas et al. The markers that mapped closest to Lr32 were Xwmc43 and Xbarc , which cosegregated 0. An unequal segregation ratio for Lr32 suggests a preference for the recovery of T.
In RL the segment introgressed from A. The separation and analysis of fluorescent fragments was done using capillary electrophoresis on an Applied Biosystems ABI genetic analyzer. Fragment sizes are reported without the bp M13 tail.
The Xwmc43 allele linked to Lr32 amplified a PCR product of bp, while the allele of the recurrent parent Thatcher had a size of bp. The bp allele was absent in a survey of common wheat lines lacking Lr32 from different origins.
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